Conserved and variant epitopes of Plasmodium vivax Duffy binding protein as 1 targets of inhibitory monoclonal antibodies
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چکیده
3 Running title: Inhibitory anti-DBP monoclonal antibodies 4 5 Francis B. Ntumngia, Jesse Schloegel, Samantha J. Barnes, Amy M. McHenry, Sanjay Singh 6 Christopher L. King , and John H. Adams 7 8 9 University of South Florida, Tampa, FL, United States 10 Case Western Reserve University, Cleveland, OH, United States 11 Veterans Affairs Medical Center, Cleveland, OH, United States 12 Gennova Biophamaceuticals Ltd, Pune, India 13 14 15 * Corresponding author. Mailing address: 3720 Spectrum Blvd., Suite 304, Tampa, FL 33612. 16 Phone: (813) 974-9916. Fax: (813) 974-0992. E-mail: [email protected] 17
منابع مشابه
Conserved and variant epitopes of Plasmodium vivax Duffy binding protein as targets of inhibitory monoclonal antibodies.
The Duffy binding protein (DBP) is a vital ligand for Plasmodium vivax blood-stage merozoite invasion, making the molecule an attractive vaccine candidate against vivax malaria. Similar to other blood-stage vaccine candidates, DBP allelic variation eliciting a strain-specific immunity may be a major challenge for development of a broadly effective vaccine against vivax malaria. To understand wh...
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Background: The purity and correct folding of a recombinant protein is critical for any structural, biochemical and vaccine design studies. Plasmodium vivax Duffy binding protein-II is a leading vaccine candidate for vivax malaria. In the present study, the purification process of recombinant DBP-IX (a variant form of PvDBP-II) was optimized to achieve the highest yield and purity. Moreover, ...
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Plasmodium vivax Duffy Binding Protein (PvDBP) is the most promising vaccine candidate for P. vivax malaria. The polymorphic nature of PvDBP induces strain-specific immune responses, however, and the epitopes of broadly neutralizing antibodies are unknown. These features hamper the rational design of potent DBP-based vaccines and necessitate the identification of globally conserved epitopes. Us...
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The Plasmodium vivax vaccine candidate Duffy Binding Protein (DBP) is a protein necessary for P. vivax invasion of reticulocytes. The polymorphic nature of DBP induces strain-specific immune responses that pose unique challenges for vaccine development. DEKnull is a synthetic DBP based antigen that has been engineered through mutation to enhance induction of blocking inhibitory antibodies. We d...
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Plasmodium vivax Duffy binding protein region II (DBPII) is an important vaccine candidate for antibody-mediated immunity against vivax malaria. A significant challenge for vaccine development of DBPII is its highly polymorphic nature that alters sensitivity to neutralizing antibody responses. Here, we aim to characterize naturally-acquired neutralizing antibodies against DBPII in individual Th...
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